The West Side Story Essay Example | Topics and Well Written Essays - 1000 words

The West Side Story - Essay Example But for everything to be alright, there was a very high price to pay, and that is Tony’s death. The West Side Story was a musical that borrowed the concept of Romeo and Juliet. It had the same plot line. There were lovers who wanted to be together despite their differences. They succeeded only to fail in living together since they died together. Of course the stories wouldn’t be that much similar or else it won’t be successful anymore. The West Side Story was a story of two rival gangs, same as Romeo and Juliet’s two rival families. It was set in Manhattan, New York. During the time the story was finalized, gang violence was rampant. It was very timely and it intrigued the public because it had a relevant social issue. The West Side Story was not just a tragedy because of the dark mood and depressing final act that it had. It was also a story about how triumph can have a very high price. In this case, it was death. With Tony’s death, the rival gangs joined together realizing what hate and anger can do to them. When Maria declared that â€Å"all of (them) killed him, not with bullets or guns, but with hate. (And now she) can kill too because now (she) has hate,† it made them realize the alarming situation of how every one of them can die because of their hatred towards each other. And this hatred is not even something that has enough bearing. They were just fighting because they had different racial backgrounds. They have different beliefs and perspectives. The hatred was passed on from one generation to another. When Anita tried to send Tony a message, she was almost raped by the Tony’s group. She was treated with disrespect and because of this, she sent out a distorted message. Instead of warning them that Chino is looking to kill Tony with a gun, she blurted out that Maria was killed by Chino in a fit of jealousy and rage. And this caused Tony to seek out Chino and his death. Anita was touched by hatred because of what was done to her. Something that could have been prevented if only she was treated with respect. But as Tony felt that it will be alright in the end, it did become alright. When the members of both gangs joined together to carry Tony’s body, it symbolizes intersecting of racial differences to repent and have peace with each other. This plot line is still powerful even up to these times. This is a story about racial differences. It tells us what biases and prejudice can do to us. It is still a significant social issue until today. It is still a challenge for some people to accept another race as part of a group or a team. Gangs are street groups. They are created to protect their territories, places where they grew up, places where they are comfortable with. Of course, as any other person, if your personal space is threatened, you fight for you. This is the same for gangs. They fight for their space. They believe that only one group can stay in one place. But because p eople cannot compromise and because hatred is set so deep in their hearts, people die. The characters in the story signify the different personas that are involved in street gangs. There are the leaders, their successors, the followers and their women. The women are not muses of the gangs. They are not direct members of the gang. They are only associated with the gang members by blood or by relationship. They may be a sister, cousin or girlfriend. But even if they are not members of the gang, they still get involved in whatever conflicts there are between the gangs. Since they are associated with the members by blood or relationship, it is believed that their loyalty will be with their own. As Chino said to Maria when her brother was killed by Tony, she should â€Å"stick with (her) own kind.† That was the thought process of someone whose racial belief is

Western Blot and its Applications Essay Example | Topics and Well Written Essays - 1000 words

Western Blot and its Applications - Essay Example Western blot analysis can detect your protein of interest from a mixture of a great number of proteins. It can give you information about the size of your protein (with comparison to a size marker or ladder in kDa), and also give you information on protein expression (with comparison to a control such as untreated sample or another cell type or tissue). It can analyze any protein sample whether from cells or tissues, but also can analyze recombinant proteins synthesized in vitro.Western blot is dependent on the quality of antibody you use to probe for your protein of interest, and how specific it is for this protein. In western blotting, proteins are electrophoresed in polyacrylamide gel, transferred onto a nitrocellulose or nylon membrane, and the protein bands are detected by their specific interaction with antibodies, lectins or some other compounds. First step involves the separation of tissue. Then proteins are separated by gel electrophoresis from sample. If you are going to western blot for protein mass you can lyse in larger volumes If you are going to western blot a phospho-protein use phosphatase inhibitors. It will remove the phosphates from your proteins. If you are looking at protein-protein interactions use a less-stringent detergent such RIPA. By far the most common type of gel electrophoresis employs polyacrylamide gels and buffers loaded with Sodium Dodecyl Sulphate (SDS). SDS PAGE allows separation of proteins by their molecular weight. Steps involved in obtaining protein for western blot. The protein bands are transferred onto a nitrocellulose or nylon membrane; initially this was achieved by a capillary movement of buffer but now a days it is usually done by electrophoresis (electrophoretic blotting). The electrophoretic blotting, both of proteins and nucleic acids are much faster and more efficient than capillary blotting. One thing to be aware of is that proteins bind better to nitrocellulose at a low pH. You may need to go through some trial-and-error to find the optimal pH. You also need to be sure there are no air bubbles between the nitrocellulose and the gel or your proteins will not transfer. The specific protein bands are identified in a variety of ways. 1. Antibodies are the most commonly used probes for detecting specific antigens. 2. Lectins are used as probes for the identification of glycoproteins. These probes may themselves be radioactive or a radioactive molecule may be tagged to them. Often the identification process is based on a 'Sandwich' reaction. In such an approach, a species specific second antibody or protein A of Staphylococcus aureus or streptavidin is used to bind to the antibodies bound to the protein bands. These second molecules may be labelled with radioactive, enzyme or fluorescent tags, Steps in conducting a western blot. a single preparation of these labelled molecules can be employed as a general detector for various probes. Applications in different fields The conformity HIV test uses a HIV western blot to detect anti- HIV. The HIV Western blot consists of a thin nitrocellulose strip in which are embedded proteins claimed to be unique to HIV. Each protein is labelled with a 'p' followed by its molecular weight in thousands. Serum is added to the strip and if there are antibodies to a particular protein this band will 'light up'. The HIV Western blot is not standardised. Lyme disease testing also uses Western blotting. The IgG Western Blot is a sandwich-type immunoassay performed in a manner that allows visualization of the patient's antibodies. It is a qualitative test and is generally more sensitive and specific than the ELISA. A positive IgG result with clinical history may be indicative of